| Name |
Apob ELISA Kit (Pig) (GWB-KBBUN9) |
| Gene Symbol |
APOB |
| Gene id |
100725937 |
| Alias Symbols |
Apo-B, FLDB, Apo B-100, Apo B-48 |
| Protein Name |
Apolipoprotein B |
| Swissprot ID |
Q29433 |
| Protein Accession Num |
XP_003473207 |
| Nucleotide Accession Num |
XM_003473159 |
| Species Reactivity |
Pig |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
< 2.82 ng/mL |
| Kit Range |
6.25 - 400 ng/mL |
| Sample Type |
serum, plasma and other biological fluids |
| Datasheets / Downloads |
GWB-KBBUN9 Datasheet |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Principle |
Genway Biotech Apob ELISA Kit (Pig) (GWB-KBBUN9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apob has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apob is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apob captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-tat Microplate | 96 Wells (12 x 8 Well Strips) | | tat Lyophilized Standard | 2 x 400 ng | | 100X Biotinylated tat Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Kit Detection |
Colorimetric, OD 450 nm |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 81-98% | 95-104% | 94-101% | 80-92% | |
| EDTA Plasma (n=5) | 79-92% | 87-94% | 83-90% | 95-105% | |
| Heparin Plasma (n=5) | 86-96% | 83-98% | 79-103% | 91-98% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 86-95 | 90 | |
| EDTA Plasma (n=5) | 92-103 | 101 | |
| Heparin Plasma (n=5) | 88-102 | 93 | |
|
