Apob ELISA Kit (Pig) (GWB-KBBUN9)

GWB-KBBUN9

96 Wells
$700.00
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Additional Information

Name Apob ELISA Kit (Pig) (GWB-KBBUN9)
Kit Range 6.25 - 400 ng/mL
Sensitivity < 2.82 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol APOB
Gene id 100725937
Alias Symbols Apo-B, FLDB, Apo B-100, Apo B-48
Swissprot ID Q29433
Protein Name Apolipoprotein B
Kit Principle Genway Biotech Apob ELISA Kit (Pig) (GWB-KBBUN9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apob has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apob is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apob captured in the well.
Kit Component
ComponentAmount
Anti-tat Microplate96 Wells (12 x 8 Well Strips)
tat Lyophilized Standard2 x 400 ng
100X Biotinylated tat Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_003473207
Nucleotide Accession Num XM_003473159
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)81-98%95-104%94-101%80-92%
EDTA Plasma (n=5)79-92%87-94%83-90%95-105%
Heparin Plasma (n=5)86-96%83-98%79-103%91-98%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-9590
EDTA Plasma (n=5)92-103101
Heparin Plasma (n=5)88-10293
Datasheets/Manuals Printable datasheet for GWB-KBBUN9