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96 Wells
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Additional Information

Description of Target May play a role in lipid transport [rgd, feb 2006]
Kit Range 0.156-10 ng/mL
Sensitivity <0.036 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant.
Kit Detection Absorbance, OD 450 nm
Gene Symbol APOC1
Gene id 25292
Alias Symbols ALPCI, ApoC-IB, Apo-CIB, Apolipoprotein C1, Apolipoprotein C-I, Liver regeneration-related protein LRRG04, LRRG04
Swissprot ID P19939
Protein Name apolipoprotein C-I
Kit Principle Genway Biotech APOC1 ELISA Kit (Rat) (GWB-KBBMF8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APOC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOC1 captured in the well.
Kit Component
Anti-APOC1 Microplate96 Wells (12 x 8 Well Strips)
APOC1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X APOC1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001103466.1
Nucleotide Accession Num NM_001109996.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMF8
Species Reactivity Rat