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AQP8 ELISA Kit (Human) (GWB-KBBLI6)

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GWB-KBBLI6

96 Wells
$659.00
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Additional Information

Name AQP8 ELISA Kit (Human) (GWB-KBBLI6)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AQP8
Gene id 343
Alias Symbols AQP-8, Aquaporin-8
Swissprot ID O94778
Protein Name aquaporin-8
Description of Target Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.
Kit Principle Genway Biotech AQP8 ELISA Kit (Human) (GWB-KBBLI6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AQP8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AQP8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AQP8 captured in the well.
Kit Component
ComponentAmount
Anti-CHRNB3 Microplate96 Wells (12 x 8 Well Strips)
CHRNB3 Lyophilized Standard2 x 20 ng
100X Biotinylated CHRNB3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001160
Nucleotide Accession Num NM_001169
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBLI6
Species Reactivity Human