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96 Wells

Additional Information

Kit Range 0.156-10 ng/mL
Sensitivity <0.064ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ARHGEF7
Gene id 114559
Alias Symbols Beta-Pix, P85spr, Pak3bp, PAK-interacting exchange factor beta, Pixb, Rho guanine nucleotide exchange factor 7
Swissprot ID O55043
Protein Name rho guanine nucleotide exchange factor 7
Description of Target Rac/cdc42-specific guanine nucleotide exchange factor, mediates bfgf-induced neurite outgrowth [rgd, feb 2006]
Kit Principle Genway Biotech ARHGEF7 ELISA Kit (Rat) (GWB-KBBDQ6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARHGEF7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARHGEF7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARHGEF7 captured in the well.
Kit Component
Anti-ARHGEF7 Microplate96 Wells (12 x 8 Well Strips)
ARHGEF7 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ARHGEF7 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001106994.1
Nucleotide Accession Num NM_001113522.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBDQ6
Species Reactivity Rat