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ARNTL ELISA Kit (Human) (GWB-KBBYF6)

GWB-KBBYF6

96 Wells
$580.00
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Additional Information

Name ARNTL ELISA Kit (Human) (GWB-KBBYF6)
Availability In Stock
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.114 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ARNTL
Gene id 406
Alias Symbols Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic-helix-loop-helix-PAS protein MOP3, bHLHe5, BHLHE5, bHLH-PAS protein JAP3, BMAL1, BMAL1c, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5, JAP3, Member of PAS prote
Swissprot ID O00327
Protein Name Aryl hydrocarbon receptor nuclear translocator-like protein 1
Description of Target Transcriptional activator which forms a core component of the circadian clock.
Kit Principle Genway Biotech ARNTL ELISA Kit (Human) (GWB-KBBYF6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARNTL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARNTL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARNTL captured in the well.
Kit Component
ComponentAmount
Anti-ARNTL Microplate96 Wells (12 x 8 Well Strips)
ARNTL Lyophilized Standard2 x 20 ng
100X Biotinylated ARNTL Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001025443
Nucleotide Accession Num NM_001030272
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBYF6
Species Reactivity Human