ASGR2 ELISA Kit (Human) (GWB-KBBYV3) (Aviva Cat. No. OKDD00135)

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GWB-KBBYV3

Aviva Cat. No.: OKDD00135

Additional Information

Name ASGR2 ELISA Kit (Human) (GWB-KBBYV3)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.049 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ASGR2
Gene id 433
Alias Symbols ASGPR2, ASGPR 2, ASGP-R2, ASGP-R 2, Asialoglycoprotein receptor 2, CLEC4H2, C-type lectin domain family 4 member H2, FLJ60040, HBXBP, Hepatic lectin H2, HL-2
Swissprot ID P07307
Protein Name asialoglycoprotein receptor 2
Description of Target This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed term
Kit Principle Genway Biotech ASGR2 ELISA Kit (Human) (GWB-KBBYV3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ASGR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ASGR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ASGR2 captured in the well.
Kit Component
ComponentAmount
Anti-ASGR2 Microplate96 Wells (12 x 8 Well Strips)
ASGR2 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ASGR2 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001172.1
Nucleotide Accession Num NM_001181.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBYV3
Species Reactivity Human