| Name |
ATG16L1 ELISA Kit (Human) (GWB-KBBID2) |
| Kit Range |
0.156 - 10 ng/mL |
| Sensitivity |
< 0.063 ng/mL |
| Sample Type |
tissue homogenates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
ATG16L1 |
| Gene id |
55054 |
| Alias Symbols |
APG16L, APG16-like 1, ATG16A, ATG16L, Autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, UNQ9393/PRO34307, WDR30 |
| Swissprot ID |
Q676U5 |
| Protein Name |
autophagy-related protein 16-1 |
| Description of Target |
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elon |
| Kit Principle |
Genway Biotech ATG16L1 ELISA Kit (Human) (GWB-KBBID2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATG16L1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATG16L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATG16L1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-MYH7B Microplate | 96 Wells (12 x 8 Well Strips) | | MYH7B Lyophilized Standard | 2 x 10 ng | | 100X Biotinylated MYH7B Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001177195 |
| Nucleotide Accession Num |
NM_001190266 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBID2 |
| Species Reactivity |
Human |
