ATG16L1 ELISA Kit (Human) (GWB-KBBID2) (Aviva Cat. No. OKCD00628)

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GWB-KBBID2

Aviva Cat. No.: OKCD00628

Additional Information

Name ATG16L1 ELISA Kit (Human) (GWB-KBBID2)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ATG16L1
Gene id 55054
Alias Symbols APG16L, APG16-like 1, ATG16A, ATG16L, Autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, UNQ9393/PRO34307, WDR30
Swissprot ID Q676U5
Protein Name autophagy-related protein 16-1
Description of Target Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elon
Kit Principle Genway Biotech ATG16L1 ELISA Kit (Human) (GWB-KBBID2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATG16L1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATG16L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATG16L1 captured in the well.
Kit Component
ComponentAmount
Anti-MYH7B Microplate96 Wells (12 x 8 Well Strips)
MYH7B Lyophilized Standard2 x 10 ng
100X Biotinylated MYH7B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001177195
Nucleotide Accession Num NM_001190266
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBID2
Species Reactivity Human