ATG16L1 ELISA Kit (Human) (GWB-KBBPT5) (Aviva Cat. No. OKDD00139)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKDD00139 to view product details and for ordering information.


GWB-KBBPT5

Aviva Cat. No.: OKDD00139

Additional Information

Name ATG16L1 ELISA Kit (Human) (GWB-KBBPT5)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ATG16L1
Gene id 55054
Alias Symbols APG16L, APG16-like 1, ATG16A, ATG16L, Autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, UNQ9393/PRO34307, WDR30
Swissprot ID Q676U5
Protein Name Autophagy-related protein 16-1
Description of Target The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to infla
Kit Principle Genway Biotech ATG16L1 ELISA Kit (Human) (GWB-KBBPT5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATG16L1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATG16L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATG16L1 captured in the well.
Kit Component
ComponentAmount
Anti-ATG16L1 Microplate96 Wells (12 x 8 Well Strips)
ATG16L1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ATG16L1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001177195.1
Nucleotide Accession Num NM_001190266.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBPT5
Species Reactivity Human