Atp1a1 ELISA Kit (Rabbit) (GWB-KBBNC5) (Aviva Cat. No. OKCD00995)

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GWB-KBBNC5

Aviva Cat. No.: OKCD00995

Additional Information

Name Atp1a1 ELISA Kit (Rabbit) (GWB-KBBNC5)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.115 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ATP1A1
Gene id 100302415
Alias Symbols Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1
Swissprot ID Q9N0Z6
Protein Name Sodium/potassium-transporting ATPase subunit alpha-1
Description of Target This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, p
Kit Principle Genway Biotech Atp1a1 ELISA Kit (Rabbit) (GWB-KBBNC5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp1a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atp1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp1a1 captured in the well.
Kit Component
ComponentAmount
Anti-BHMT Microplate96 Wells (12 x 8 Well Strips)
BHMT Lyophilized Standard2 x 20 ng
100X Biotinylated BHMT Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001156546
Nucleotide Accession Num NM_001163074
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBNC5
Species Reactivity Rabbit