| Name |
Atp1a1 ELISA Kit (Rabbit) (GWB-KBBNC5) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.115 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
ATP1A1 |
| Gene id |
100302415 |
| Alias Symbols |
Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1 |
| Swissprot ID |
Q9N0Z6 |
| Protein Name |
Sodium/potassium-transporting ATPase subunit alpha-1 |
| Description of Target |
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, p |
| Kit Principle |
Genway Biotech Atp1a1 ELISA Kit (Rabbit) (GWB-KBBNC5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp1a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atp1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp1a1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-BHMT Microplate | 96 Wells (12 x 8 Well Strips) | | BHMT Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated BHMT Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001156546 |
| Nucleotide Accession Num |
NM_001163074 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBNC5 |
| Species Reactivity |
Rabbit |
