| Name |
ATP1a1 ELISA Kit (Rat) (GWB-KBBTC7) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
<0.062ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
ATP1a1 |
| Gene id |
24211 |
| Alias Symbols |
Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1 |
| Swissprot ID |
P06685 |
| Protein Name |
sodium/potassium-transporting ATPase subunit alpha-1 |
| Description of Target |
Mediates na+ and k+ transport, may play a role in regulation of blood pressure [rgd, feb 2006] |
| Kit Principle |
Genway Biotech ATP1a1 ELISA Kit (Rat) (GWB-KBBTC7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP1a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP1a1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-ATP1a1 Microplate | 96 Wells (12 x 8 Well Strips) |
| ATP1a1 Lyophilized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X ATP1a1 Biotinylated Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_036636.1 |
| Nucleotide Accession Num |
NM_012504.1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBTC7 |
| Species Reactivity |
Rat |
