ATP1a1 ELISA Kit (Rat) (GWB-KBBTC7) (Aviva Cat. No. OKDD00804)

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GWB-KBBTC7

Aviva Cat. No.: OKDD00804

Additional Information

Name ATP1a1 ELISA Kit (Rat) (GWB-KBBTC7)
Kit Range 0.156-10 ng/mL
Sensitivity <0.062ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ATP1a1
Gene id 24211
Alias Symbols Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1
Swissprot ID P06685
Protein Name sodium/potassium-transporting ATPase subunit alpha-1
Description of Target Mediates na+ and k+ transport, may play a role in regulation of blood pressure [rgd, feb 2006]
Kit Principle Genway Biotech ATP1a1 ELISA Kit (Rat) (GWB-KBBTC7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP1a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP1a1 captured in the well.
Kit Component
ComponentAmount
Anti-ATP1a1 Microplate96 Wells (12 x 8 Well Strips)
ATP1a1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ATP1a1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_036636.1
Nucleotide Accession Num NM_012504.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTC7
Species Reactivity Rat