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Additional Information

Name ATP2A2 ELISA Kit (Human) (GWB-KBBCT4)
Description of Target This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle Acts as a regulator
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ATP2A2
Gene id 488
Alias Symbols ATP2B, Calcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, DAR, DD, DKFZp686P0211, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, FLJ20293, FLJ38063, MGC45367, Sarcoplasmic/endoplasmic reticulum ca
Swissprot ID P16615
Protein Name sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Kit Principle Genway Biotech ATP2A2 ELISA Kit (Human) (GWB-KBBCT4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP2A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP2A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP2A2 captured in the well.
Kit Component
Anti-Urod Microplate96 Wells (12 x 8 Well Strips)
Urod Lyophilized Standard2 x 20 ng
100X Biotinylated Urod Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001672
Nucleotide Accession Num NM_001681
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBCT4
Species Reactivity Human