ATP4a ELISA Kit (Human) (GWB-KBBOY2)

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GWB-KBBOY2

96 Wells
$530.00
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Additional Information

Name ATP4a ELISA Kit (Human) (GWB-KBBOY2)
Description of Target The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This
Kit Range 0.156-10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ATP4a
Gene id 495
Alias Symbols ATP6A, Gastric H(+)/K(+) ATPase subunit alpha, Potassium-transporting ATPase alpha chain 1, Proton pump
Swissprot ID P20648
Protein Name potassium-transporting ATPase alpha chain 1
Kit Principle Genway Biotech ATP4a ELISA Kit (Human) (GWB-KBBOY2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP4a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP4a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP4a captured in the well.
Kit Component
ComponentAmount
Anti-ATP4a Microplate96 Wells (12 x 8 Well Strips)
ATP4a Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ATP4a Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000695.2
Nucleotide Accession Num NM_000704.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBOY2
Species Reactivity Human