ATP4a ELISA Kit (Mouse) (GWB-KBBUC4)

GWB-KBBUC4

96 Wells
$555.00
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Additional Information

Name ATP4a ELISA Kit (Mouse) (GWB-KBBUC4)
Kit Range 0.156-10 ng/mL
Sensitivity <0.055 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ATP4a
Gene id 11944
Swissprot ID E9QNX7
Protein Name potassium-transporting ATPase alpha chain 1
Kit Principle Genway Biotech ATP4a ELISA Kit (Mouse) (GWB-KBBUC4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP4a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP4a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP4a captured in the well.
Kit Component
ComponentAmount
Anti-ATP4a Microplate96 Wells (12 x 8 Well Strips)
ATP4a Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ATP4a Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001277556.1
Nucleotide Accession Num NM_001290627.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Datasheets / Downloads GWB-KBBUC4 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours