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ATP7b ELISA Kit (Human) (GWB-KBBKA6)

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96 Wells
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Additional Information

Name ATP7b ELISA Kit (Human) (GWB-KBBKA6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.056 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ATP7b
Gene id 540
Alias Symbols Copper pump 2, Copper-transporting ATPase 2, PWD, WC1, WD, Wilson disease-associated protein, WND
Swissprot ID P35670
Protein Name Copper-transporting ATPase 2
Kit Principle Genway Biotech ATP7b ELISA Kit (Human) (GWB-KBBKA6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP7b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP7b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP7b captured in the well.
Kit Component
Anti-ATP7b Microplate96 Wells (12 x 8 Well Strips)
ATP7b Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ATP7b Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000044.2
Nucleotide Accession Num NM_000053.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBKA6
Species Reactivity Human