ATP7B ELISA Kit (Human) (GWB-KBBST9)

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GWB-KBBST9

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Additional Information

Name ATP7B ELISA Kit (Human) (GWB-KBBST9)
Description of Target Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.056 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ATP7B
Gene id 540
Alias Symbols Copper pump 2, Copper-transporting ATPase 2, PWD, WC1, WD, Wilson disease-associated protein, WND
Swissprot ID P35670
Protein Name copper-transporting ATPase 2
Kit Principle Genway Biotech ATP7B ELISA Kit (Human) (GWB-KBBST9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP7B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP7B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP7B captured in the well.
Kit Component
ComponentAmount
Anti-DSC1 Microplate96 Wells (12 x 8 Well Strips)
DSC1 Lyophilized Standard2 x 10 ng
100X Biotinylated DSC1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000044
Nucleotide Accession Num NM_000053
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBST9
Species Reactivity Human