Avp ELISA Kit (Mouse) (GWB-KBBRG2)

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GWB-KBBRG2

96 Wells
$629.00
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Additional Information

Name Avp ELISA Kit (Mouse) (GWB-KBBRG2)
Description of Target Neurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 5.8 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AVP
Gene id 11998
Alias Symbols AVP-NPII, Vasopressin-neurophysin 2-copeptin, Vp, Vsp
Swissprot ID P35455
Protein Name vasopressin-neurophysin 2-copeptin
Kit Principle Genway Biotech Avp ELISA Kit (Mouse) (GWB-KBBRG2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Avp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Avp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Avp captured in the well.
Kit Component
ComponentAmount
Anti-Avp Microplate96 Wells (12 x 8 Well Strips)
Avp Lyophilized Standard2 x 1 ng
100X Biotinylated Avp Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_033862
Nucleotide Accession Num NM_009732
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%92-104%90-103%96-103%
EDTA Plasma (n=5)80-99%78-101%81-89%93-105%
Heparin Plasma (n=5)88-97%83-97%87-96%97-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)95-103101
EDTA Plasma (n=5)87-10298
Heparin Plasma (n=5)96-10499
Datasheets/Manuals Printable datasheet for GWB-KBBRG2
Species Reactivity Mouse