B3galt2 ELISA Kit (Mouse) (GWB-KBBZX6)

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GWB-KBBZX6

96 Wells
$689.00
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Additional Information

Name B3galt2 ELISA Kit (Mouse) (GWB-KBBZX6)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.073 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol B3GALT2
Gene id 26878
Alias Symbols Beta-1,3-galactosyltransferase 2, Beta-1,3-GalTase 2, Beta3GalT2, Beta3Gal-T2, UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase-II
Swissprot ID O54905
Protein Name Beta-1,3-galactosyltransferase 2
Description of Target Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Can also utilize substrates with a terminal galactose residue, albeit with lower efficiency. Involved
Kit Principle Genway Biotech B3galt2 ELISA Kit (Mouse) (GWB-KBBZX6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B3galt2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B3galt2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B3galt2 captured in the well.
Kit Component
ComponentAmount
Anti-B3galt2 Microplate96 Wells (12 x 8 Well Strips)
B3galt2 Lyophilized Standard2 x 10 ng
100X Biotinylated B3galt2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_064409
Nucleotide Accession Num NM_020025
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-94%84-92%99-105%92-105%
EDTA Plasma (n=5)81-105%89-102%94-101%84-95%
Heparin Plasma (n=5)94-102%78-95%84-92%88-104%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9789
EDTA Plasma (n=5)83-10392
Heparin Plasma (n=5)81-9587
Datasheets/Manuals Printable datasheet for GWB-KBBZX6
Species Reactivity Mouse