BAI1 ELISA Kit (Human) (GWB-KBBDH3) (Aviva Cat. No. OKCD01663)

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Please see item number OKCD01663 to view product details and for ordering information.


GWB-KBBDH3

Aviva Cat. No.: OKCD01663

Additional Information

Name BAI1 ELISA Kit (Human) (GWB-KBBDH3)
Kit Range 31.2 - 2,000 pg/mL
Sensitivity < 13.1 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BAI1
Gene id 575
Alias Symbols Brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF
Swissprot ID O14514
Protein Name Brain-specific angiogenesis inhibitor 1
Description of Target Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Likely to be a potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53 signal in suppression of glioblastoma. May function in cell adhe
Kit Principle Genway Biotech BAI1 ELISA Kit (Human) (GWB-KBBDH3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BAI1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BAI1 captured in the well.
Kit Component
ComponentAmount
Anti-BAI1 Microplate96 Wells (12 x 8 Well Strips)
BAI1 Lyophilized Standard2 x 2 ng
100X Biotinylated BAI1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001693
Nucleotide Accession Num NM_001702
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)83-105%78-99%80-95%85-99%
EDTA Plasma (n=5)79-92%80-96%95-102%87-101%
Heparin Plasma (n=5)91-101%88-102%84-96%81-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-105101
EDTA Plasma (n=5)87-9892
Heparin Plasma (n=5)87-9692
Datasheets/Manuals Printable datasheet for GWB-KBBDH3
Species Reactivity Human