| Name |
BAMBI ELISA Kit (Human) (GWB-KBBGK7) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.064 ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates, cell lysates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
BAMBI |
| Gene id |
25805 |
| Alias Symbols |
BMP and activin membrane-bound inhibitor homolog, NMA, Non-metastatic gene A protein |
| Swissprot ID |
Q13145 |
| Protein Name |
BMP and activin membrane-bound inhibitor homolog |
| Description of Target |
This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encode |
| Kit Principle |
Genway Biotech BAMBI ELISA Kit (Human) (GWB-KBBGK7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BAMBI has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAMBI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BAMBI captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-BAMBI Microplate | 96 Wells (12 x 8 Well Strips) |
| BAMBI Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X BAMBI Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Protein Accession Num |
NP_036474.1 |
| Nucleotide Accession Num |
NM_012342.2 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBGK7 |
| Species Reactivity |
Human |
