BAMBI ELISA Kit (Human) (GWB-KBBGK7) (Aviva Cat. No. OKDD00145)

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GWB-KBBGK7

Aviva Cat. No.: OKDD00145

Additional Information

Name BAMBI ELISA Kit (Human) (GWB-KBBGK7)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.064 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol BAMBI
Gene id 25805
Alias Symbols BMP and activin membrane-bound inhibitor homolog, NMA, Non-metastatic gene A protein
Swissprot ID Q13145
Protein Name BMP and activin membrane-bound inhibitor homolog
Description of Target This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encode
Kit Principle Genway Biotech BAMBI ELISA Kit (Human) (GWB-KBBGK7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BAMBI has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAMBI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BAMBI captured in the well.
Kit Component
ComponentAmount
Anti-BAMBI Microplate96 Wells (12 x 8 Well Strips)
BAMBI Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X BAMBI Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_036474.1
Nucleotide Accession Num NM_012342.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBGK7
Species Reactivity Human