BAMBI ELISA Kit (Human) (GWB-KBBWJ2) (Aviva Cat. No. OKCD00570)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00570 to view product details and for ordering information.


GWB-KBBWJ2

Aviva Cat. No.: OKCD00570

Additional Information

Name BAMBI ELISA Kit (Human) (GWB-KBBWJ2)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BAMBI
Gene id 25805
Alias Symbols BMP and activin membrane-bound inhibitor homolog, NMA, Non-metastatic gene A protein
Swissprot ID Q13145
Protein Name BMP and activin membrane-bound inhibitor homolog
Description of Target Negatively regulates TGF-beta signaling.
Kit Principle Genway Biotech BAMBI ELISA Kit (Human) (GWB-KBBWJ2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BAMBI has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAMBI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BAMBI captured in the well.
Kit Component
ComponentAmount
Anti-ASTL Microplate96 Wells (12 x 8 Well Strips)
ASTL Lyophilized Standard2 x 10 ng
100X Biotinylated ASTL Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_036474
Nucleotide Accession Num NM_012342
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBWJ2
Species Reactivity Human