Bche ELISA Kit (Rat) (GWB-KBBQG5) (Aviva Cat. No. OKCD00683)

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Please see item number OKCD00683 to view product details and for ordering information.


GWB-KBBQG5

Aviva Cat. No.: OKCD00683

Additional Information

Name Bche ELISA Kit (Rat) (GWB-KBBQG5)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.63 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BCHE
Gene id 65036
Alias Symbols BuChE, CHE1, E1, Pseudocholinesterase, Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II
Swissprot ID Q9JKC1
Protein Name Carboxylic ester hydrolase
Kit Principle Genway Biotech Bche ELISA Kit (Rat) (GWB-KBBQG5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bche has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bche is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bche captured in the well.
Kit Component
ComponentAmount
Anti-LACRT Microplate96 Wells (12 x 8 Well Strips)
LACRT Lyophilized Standard2 x 100 ng
100X Biotinylated LACRT Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_075231
Nucleotide Accession Num NM_022942
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-104%91-99%98-105%94-101%
EDTA Plasma (n=5)91-101%93-101%89-97%80-92%
Heparin Plasma (n=5)82-101%78-105%82-91%80-93%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)89-9693
EDTA Plasma (n=5)86-10394
Heparin Plasma (n=5)81-9691
Datasheets/Manuals Printable datasheet for GWB-KBBQG5
Species Reactivity Rat