| Name |
Bche ELISA Kit (Rat) (GWB-KBBQG5) |
| Kit Range |
1.56 - 100 ng/mL |
| Sensitivity |
< 0.63 ng/mL |
| Sample Type |
serum, plasma, tissue homogenates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
BCHE |
| Gene id |
65036 |
| Alias Symbols |
BuChE, CHE1, E1, Pseudocholinesterase, Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II |
| Swissprot ID |
Q9JKC1 |
| Protein Name |
Carboxylic ester hydrolase |
| Kit Principle |
Genway Biotech Bche ELISA Kit (Rat) (GWB-KBBQG5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bche has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bche is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bche captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-LACRT Microplate | 96 Wells (12 x 8 Well Strips) | | LACRT Lyophilized Standard | 2 x 100 ng | | 100X Biotinylated LACRT Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_075231 |
| Nucleotide Accession Num |
NM_022942 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 80-104% | 91-99% | 98-105% | 94-101% | |
| EDTA Plasma (n=5) | 91-101% | 93-101% | 89-97% | 80-92% | |
| Heparin Plasma (n=5) | 82-101% | 78-105% | 82-91% | 80-93% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 89-96 | 93 | |
| EDTA Plasma (n=5) | 86-103 | 94 | |
| Heparin Plasma (n=5) | 81-96 | 91 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBQG5 |
| Species Reactivity |
Rat |
