This exact product is now provided by Aviva Systems Biology.
Please visit OKDD00147 to review further and for ordering information.


96 Wells

Additional Information

Kit Range 0.156-10 ng/mL
Sensitivity < 0.043 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol BCKDK
Gene id 29603
Alias Symbols BCKDHKIN, BCKD-kinase, Branched-chain alpha-ketoacid dehydrogenase kinase
Swissprot ID Q00972
Protein Name [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
Description of Target mitochondrial kinase; involved in oxidative degradation of branched-chain amino acids [RGD, Feb 2006]
Kit Principle Genway Biotech BCKDK ELISA Kit (Rat) (GWB-KBBIA4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCKDK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCKDK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCKDK captured in the well.
Kit Component
Anti-BCKDK Microplate96 Wells (12 x 8 Well Strips)
BCKDK Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X BCKDK Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_062117.2
Nucleotide Accession Num NM_019244.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIA4
Species Reactivity Rat