Bckdk ELISA Kit (Rat) (GWB-KBBTT2)

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96 Wells
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Additional Information

Name Bckdk ELISA Kit (Rat) (GWB-KBBTT2)
Kit Range 3.12 - 200 ng/mL
Sensitivity < 1.29 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BCKDK
Gene id 29603
Alias Symbols BCKDHKIN, BCKD-kinase, Branched-chain alpha-ketoacid dehydrogenase kinase
Swissprot ID Q00972
Protein Name [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
Description of Target Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex
Kit Principle Genway Biotech Bckdk ELISA Kit (Rat) (GWB-KBBTT2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bckdk has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bckdk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bckdk captured in the well.
Kit Component
Anti-Ppbp Microplate96 Wells (12 x 8 Well Strips)
Ppbp Lyophilized Standard2 x 200 ng
100X Biotinylated Ppbp Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_062117
Nucleotide Accession Num NM_019244
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTT2
Species Reactivity Rat