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BCL2L11 ELISA Kit (Human) (GWB-KBBMO2)

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Additional Information

Name BCL2L11 ELISA Kit (Human) (GWB-KBBMO2)
Description of Target Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induce
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BCL2L11
Gene id 10018
Alias Symbols BAM, Bcl2-interacting mediator of cell death, Bcl2-L-11, Bcl-2-like protein 11, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BimEL, BimL, BOD
Swissprot ID O43521
Protein Name Bcl-2-like protein 11
Kit Principle Genway Biotech BCL2L11 ELISA Kit (Human) (GWB-KBBMO2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL2L11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL2L11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL2L11 captured in the well.
Kit Component
Anti-BCL2L11 Microplate96 Wells (12 x 8 Well Strips)
BCL2L11 Lyophilized Standard2 x 20 ng
100X Biotinylated BCL2L11 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001191035
Nucleotide Accession Num NM_001204106
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMO2
Species Reactivity Human