BCL9L ELISA Kit (Human) (GWB-KBBVZ3) (Aviva Cat. No. OKCD01825)

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Aviva Cat. No.: OKCD01825

Additional Information

Name BCL9L ELISA Kit (Human) (GWB-KBBVZ3)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.124 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BCL9L
Gene id 283149
Alias Symbols B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-2, BCL9-like protein, DLNB11, Protein BCL9-2
Swissprot ID Q86UU0
Protein Name B-cell CLL/lymphoma 9-like protein
Description of Target Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1.
Kit Principle Genway Biotech BCL9L ELISA Kit (Human) (GWB-KBBVZ3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL9L has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL9L is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL9L captured in the well.
Kit Component
Anti-BCL9L Microplate96 Wells (12 x 8 Well Strips)
BCL9L Lyophilized Standard2 x 20 ng
100X Biotinylated BCL9L Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_872363
Nucleotide Accession Num NM_182557
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBVZ3
Species Reactivity Human