Bdnf ELISA Kit (Guinea Pig) (GWB-KBBHH3) (Aviva Cat. No. OKCD00213)

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Please see item number OKCD00213 to view product details and for ordering information.


GWB-KBBHH3

Aviva Cat. No.: OKCD00213

Additional Information

Name Bdnf ELISA Kit (Guinea Pig) (GWB-KBBHH3)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BDNF
Gene id 100730257
Alias Symbols Neurotrophin, Abrineurin
Swissprot ID O70183
Description of Target During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regula
Kit Principle Genway Biotech Bdnf ELISA Kit (Guinea Pig) (GWB-KBBHH3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bdnf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bdnf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bdnf captured in the well.
Kit Component
ComponentAmount
Anti-Cxcr1 Microplate96 Wells (12 x 8 Well Strips)
Cxcr1 Lyophilized Standard2 x 10 ng
100X Biotinylated Cxcr1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_003465057
Nucleotide Accession Num XM_003465009
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-99%88-102%80-105%88-102%
EDTA Plasma (n=5)78-88%96-103%93-101%88-95%
Heparin Plasma (n=5)78-89%82-93%88-97%78-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)85-10291
EDTA Plasma (n=5)90-9793
Heparin Plasma (n=5)92-9996
Datasheets/Manuals Printable datasheet for GWB-KBBHH3
Species Reactivity Guinea Pig