| Name |
Bdnf ELISA Kit (Guinea Pig) (GWB-KBBHH3) |
| Kit Range |
0.156 - 10 ng/mL |
| Sensitivity |
< 0.059 ng/mL |
| Sample Type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
BDNF |
| Gene id |
100730257 |
| Alias Symbols |
Neurotrophin, Abrineurin |
| Swissprot ID |
O70183 |
| Description of Target |
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regula |
| Kit Principle |
Genway Biotech Bdnf ELISA Kit (Guinea Pig) (GWB-KBBHH3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bdnf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bdnf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bdnf captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-Cxcr1 Microplate | 96 Wells (12 x 8 Well Strips) | | Cxcr1 Lyophilized Standard | 2 x 10 ng | | 100X Biotinylated Cxcr1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
XP_003465057 |
| Nucleotide Accession Num |
XM_003465009 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 85-99% | 88-102% | 80-105% | 88-102% | |
| EDTA Plasma (n=5) | 78-88% | 96-103% | 93-101% | 88-95% | |
| Heparin Plasma (n=5) | 78-89% | 82-93% | 88-97% | 78-102% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 85-102 | 91 | |
| EDTA Plasma (n=5) | 90-97 | 93 | |
| Heparin Plasma (n=5) | 92-99 | 96 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBHH3 |
| Species Reactivity |
Guinea Pig |
