Bglap ELISA Kit (Rabbit) (GWB-KBBKD2) (Aviva Cat. No. OKCD00800)

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Please see item number OKCD00800 to view product details and for ordering information.


GWB-KBBKD2

Aviva Cat. No.: OKCD00800

Additional Information

Name Bglap ELISA Kit (Rabbit) (GWB-KBBKD2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.116 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BGLAP
Gene id 100340503
Alias Symbols BGLAP, OT, BGP, Bone Gla Protein, Bone Gamma-Carboxyglutamate Protein, Gamma-carboxyglutamic acid-containing protein
Swissprot ID G1TTE8
Protein Name bone gamma-carboxyglutamate protein
Description of Target Binds strongly to apatite and calcium.
Kit Principle Genway Biotech Bglap ELISA Kit (Rabbit) (GWB-KBBKD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bglap has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bglap is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bglap captured in the well.
Kit Component
ComponentAmount
Anti-PKN1 Microplate96 Wells (12 x 8 Well Strips)
PKN1 Lyophilized Standard2 x 20 ng
100X Biotinylated PKN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_002715429  
Nucleotide Accession Num XM_002715383
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-91%96-104%90-104%93-101%
EDTA Plasma (n=5)91-101%80-92%90-105%84-98%
Heparin Plasma (n=5)79-101%98-105%78-90%82-92%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)84-9690
EDTA Plasma (n=5)83-9187
Heparin Plasma (n=5)82-10197
Datasheets/Manuals Printable datasheet for GWB-KBBKD2
Species Reactivity Rabbit