| Name |
Bglap ELISA Kit (Rabbit) (GWB-KBBKD2) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.116 ng/mL |
| Sample Type |
serum, plasma, tissue homogenates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
BGLAP |
| Gene id |
100340503 |
| Alias Symbols |
BGLAP, OT, BGP, Bone Gla Protein, Bone Gamma-Carboxyglutamate Protein, Gamma-carboxyglutamic acid-containing protein |
| Swissprot ID |
G1TTE8 |
| Protein Name |
bone gamma-carboxyglutamate protein |
| Description of Target |
Binds strongly to apatite and calcium. |
| Kit Principle |
Genway Biotech Bglap ELISA Kit (Rabbit) (GWB-KBBKD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bglap has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bglap is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bglap captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-PKN1 Microplate | 96 Wells (12 x 8 Well Strips) | | PKN1 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated PKN1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
XP_002715429 |
| Nucleotide Accession Num |
XM_002715383 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 80-91% | 96-104% | 90-104% | 93-101% | |
| EDTA Plasma (n=5) | 91-101% | 80-92% | 90-105% | 84-98% | |
| Heparin Plasma (n=5) | 79-101% | 98-105% | 78-90% | 82-92% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 84-96 | 90 | |
| EDTA Plasma (n=5) | 83-91 | 87 | |
| Heparin Plasma (n=5) | 82-101 | 97 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBKD2 |
| Species Reactivity |
Rabbit |
