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96 Wells

Additional Information

Name BHLHB8 ELISA Kit (Human) (GWB-KBBPU2)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.071 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol BHLHB8
Gene id 168620
Alias Symbols bHLHa15, bHLHb8, BHLHB8, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, MIST1, MIST-1, Muscle, intestine and stomach expression 1
Swissprot ID Q7RTS1
Protein Name class A basic helix-loop-helix protein 15
Kit Principle Genway Biotech BHLHB8 ELISA Kit (Human) (GWB-KBBPU2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BHLHB8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BHLHB8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BHLHB8 captured in the well.
Kit Component
Anti-BHLHB8 Microplate96 Wells (12 x 8 Well Strips)
BHLHB8 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X BHLHB8 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_803238.1
Nucleotide Accession Num NM_177455.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBPU2
Species Reactivity Human