BLK ELISA Kit (Human) (GWB-KBBGX1)

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GWB-KBBGX1

96 Wells
$659.00
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Additional Information

Name BLK ELISA Kit (Human) (GWB-KBBGX1)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.071 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BLK
Gene id 640
Alias Symbols B lymphocyte kinase, MGC10442, MODY11, p55-Blk, Tyrosine-protein kinase Blk
Swissprot ID P51451
Protein Name tyrosine-protein kinase Blk
Description of Target Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of anti
Kit Principle Genway Biotech BLK ELISA Kit (Human) (GWB-KBBGX1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BLK has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BLK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BLK captured in the well.
Kit Component
ComponentAmount
Anti-Neu2 Microplate96 Wells (12 x 8 Well Strips)
Neu2 Lyophilized Standard2 x 10 ng
100X Biotinylated Neu2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001706
Nucleotide Accession Num NM_001715
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBGX1
Species Reactivity Human