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96 Wells

Additional Information

Name BLK ELISA Kit (Human) (GWB-KBBYJ8)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.071 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol BLK
Gene id 640
Alias Symbols B lymphocyte kinase, MGC10442, MODY11, p55-Blk, Tyrosine-protein kinase Blk
Swissprot ID P51451
Protein Name Tyrosine-protein kinase Blk
Kit Principle Genway Biotech BLK ELISA Kit (Human) (GWB-KBBYJ8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BLK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BLK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BLK captured in the well.
Kit Component
Anti-BLK Microplate96 Wells (12 x 8 Well Strips)
BLK Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X BLK Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001706.2
Nucleotide Accession Num NM_001715.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBYJ8
Species Reactivity Human