BMI1 ELISA Kit (Human) (GWB-KBBZB2)

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GWB-KBBZB2

96 Wells
$659.00
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Additional Information

Name BMI1 ELISA Kit (Human) (GWB-KBBZB2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.122 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BMI1
Gene id 100532731
Alias Symbols FLVI2/BMI1, MGC12685, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, RNF51
Swissprot ID P35226
Protein Name polycomb complex protein BMI-1
Description of Target Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and
Kit Principle Genway Biotech BMI1 ELISA Kit (Human) (GWB-KBBZB2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMI1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMI1 captured in the well.
Kit Component
ComponentAmount
Anti-TRPC6 Microplate96 Wells (12 x 8 Well Strips)
TRPC6 Lyophilized Standard2 x 20 ng
100X Biotinylated TRPC6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001190991
Nucleotide Accession Num NM_001204062
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBZB2
Species Reactivity Human