|Amino Acid Sequence
||MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC CPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.
||The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 μg/ml.
||Protein quantitation was carried out by two independent methods
- UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
- Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 100mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton.
The BMP-2 is purified by proprietary chromatographic techniques. BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.
||Induces cartilage and bone formation.
||Homodimer; disulfide-linked. Interacts with GREM2 (By similarity) and SOSTDC1.
||Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
||Belongs to the TGF-beta family.