BMP3 ELISA Kit (Human) (GWB-KBBRK7) (Aviva Cat. No. OKDD00152)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKDD00152 to view product details and for ordering information.


GWB-KBBRK7

Aviva Cat. No.: OKDD00152

Additional Information

Name BMP3 ELISA Kit (Human) (GWB-KBBRK7)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol BMP3
Gene id 651
Alias Symbols BMP-3, BMP3A, BMP-3A, Bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin
Swissprot ID P12645
Protein Name bone morphogenetic protein 3
Description of Target This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate
Kit Principle Genway Biotech BMP3 ELISA Kit (Human) (GWB-KBBRK7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMP3 captured in the well.
Kit Component
ComponentAmount
Anti-BMP3 Microplate96 Wells (12 x 8 Well Strips)
BMP3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X BMP3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001192.2
Nucleotide Accession Num NM_001201.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRK7
Species Reactivity Human