BMPR1B ELISA Kit (Human) (GWB-KBBXJ4) (Aviva Cat. No. OKCD01535)

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GWB-KBBXJ4

Aviva Cat. No.: OKCD01535

Additional Information

Name BMPR1B ELISA Kit (Human) (GWB-KBBXJ4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.117 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BMPR1B
Gene id 658
Alias Symbols ALK6, ALK-6, BMPR-1B, BMP type-1B receptor, Bone morphogenetic protein receptor type-1B, CDw293
Swissprot ID O00238
Protein Name Bone morphogenetic protein receptor type-1B
Description of Target On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional
Kit Principle Genway Biotech BMPR1B ELISA Kit (Human) (GWB-KBBXJ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMPR1B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMPR1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMPR1B captured in the well.
Kit Component
ComponentAmount
Anti-BMPR1B Microplate96 Wells (12 x 8 Well Strips)
BMPR1B Lyophilized Standard2 x 20 ng
100X Biotinylated BMPR1B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001194
Nucleotide Accession Num NM_001203
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXJ4
Species Reactivity Human