BMPR2 ELISA Kit (Human) (GWB-KBBUQ1) (Aviva Cat. No. OKCD00783)

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Please see item number OKCD00783 to view product details and for ordering information.


GWB-KBBUQ1

Aviva Cat. No.: OKCD00783

Additional Information

Name BMPR2 ELISA Kit (Human) (GWB-KBBUQ1)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.124 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BMPR2
Gene id 659
Alias Symbols BMPR-2, BMPR3, BMPR-II, BMP type-2 receptor, BMP type II receptor, BMR2, Bone morphogenetic protein receptor type-2, Bone morphogenetic protein receptor type II, BRK-3, FLJ41585, FLJ76945, PPH1, T-ALK
Swissprot ID Q13873
Protein Name Bone morphogenetic protein receptor type-2
Description of Target On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional
Kit Principle Genway Biotech BMPR2 ELISA Kit (Human) (GWB-KBBUQ1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMPR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMPR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMPR2 captured in the well.
Kit Component
ComponentAmount
Anti-MMP1 Microplate96 Wells (12 x 8 Well Strips)
MMP1 Lyophilized Standard2 x 20 ng
100X Biotinylated MMP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001195
Nucleotide Accession Num NM_001204
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
83-104%83-94%80-92%86-95%
Kit Recovery
MatrixRecovery Range (%)Average (%)
94-10298
Datasheets/Manuals Printable datasheet for GWB-KBBUQ1
Species Reactivity Human