| Name |
BMPR2 ELISA Kit (Human) (GWB-KBBUQ1) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.124 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
BMPR2 |
| Gene id |
659 |
| Alias Symbols |
BMPR-2, BMPR3, BMPR-II, BMP type-2 receptor, BMP type II receptor, BMR2, Bone morphogenetic protein receptor type-2, Bone morphogenetic protein receptor type II, BRK-3, FLJ41585, FLJ76945, PPH1, T-ALK |
| Swissprot ID |
Q13873 |
| Protein Name |
Bone morphogenetic protein receptor type-2 |
| Description of Target |
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional |
| Kit Principle |
Genway Biotech BMPR2 ELISA Kit (Human) (GWB-KBBUQ1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMPR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMPR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMPR2 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-MMP1 Microplate | 96 Wells (12 x 8 Well Strips) | | MMP1 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated MMP1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001195 |
| Nucleotide Accession Num |
NM_001204 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| 83-104% | 83-94% | 80-92% | 86-95% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| 94-102 | 98 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBUQ1 |
| Species Reactivity |
Human |
