Bovine Haptoglobin ELISA Kit (GWB-A43096) (Aviva Cat. No. OKIA00002)

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GWB-A43096

Aviva Cat. No.: OKIA00002

40-374-130001

Description

Short Description: In this assay the HAPTOGLOBIN present in serum sample reacts with the anti-HAPTOGLOBIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information

Name Bovine Haptoglobin ELISA Kit (GWB-A43096)
Related Product Names HP2-ALPHA; Bovine Haptoglobin ELISA Kit; N/A Bovine HaptoglobinHP
Kit Range 15.6 ng/ml- 1,000 ng/ml
Sensitivity 6.258 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene id 280692
Alias Symbols Haptoglobin, HP, Zonulin
Swissprot ID Q2TBU0
Protein Name Haptoglobin
Description of Target As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also a
Kit Principle Genway Biotech Haptoglobin ELISA Kit (Bovine) (GWB-A43096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well.
Kit Component
ComponentAmount
Anti-Bovine Haptoglobin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Bovine Haptoglobin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Cow
NCBI Acc Number NP_001119574.1
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira┬Čtion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Hp conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-BOVINE Haptoglobin ELISA MICRO PLATE
Anti-Bovine Hp coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. BOVINE Haptoglobin CALIBRATOR
The lyophilized Bovine Haptoglobin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Bovine
Kit Size 96 well
Kit Duration 40 Minutes
Datasheets/Manuals Printable datasheet for GWB-A43096
COA Datasheet Printable COA datasheet for GWB-A43096
Storage Refer to kit instructions for component storage
Species Reactivity Cow
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Haptoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological samples of cattle.