Description
Short Description: In this assay the HAPTOGLOBIN present in serum sample reacts with the anti-HAPTOGLOBIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.
Additional Information
| Name | Bovine Haptoglobin ELISA Kit (GWB-A43096) | ||||||||||||||||
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| Related Product Names | HP2-ALPHA; Bovine Haptoglobin ELISA Kit; N/A Bovine HaptoglobinHP | ||||||||||||||||
| Kit Range | 15.6 ng/ml- 1,000 ng/ml | ||||||||||||||||
| Sensitivity | 6.258 ng/ml | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Gene id | 280692 | ||||||||||||||||
| Alias Symbols | Haptoglobin, HP, Zonulin | ||||||||||||||||
| Swissprot ID | Q2TBU0 | ||||||||||||||||
| Protein Name | Haptoglobin | ||||||||||||||||
| Description of Target | As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also a | ||||||||||||||||
| Kit Principle | Genway Biotech Haptoglobin ELISA Kit (Bovine) (GWB-A43096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well. | ||||||||||||||||
| Kit Component |
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| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Homology | Cow | ||||||||||||||||
| NCBI Acc Number | NP_001119574.1 | ||||||||||||||||
| Standard Range | Refer To Manual | ||||||||||||||||
| Product Format | In Solution | ||||||||||||||||
| Specimen/Volumes | Refer To Manual | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-Hp conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-BOVINE Haptoglobin ELISA MICRO PLATE Anti-Bovine Hp coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. BOVINE Haptoglobin CALIBRATOR The lyophilized Bovine Haptoglobin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
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| Reactivity | Bovine | ||||||||||||||||
| Kit Size | 96 well | ||||||||||||||||
| Kit Duration | 40 Minutes | ||||||||||||||||
| Datasheets/Manuals | Printable datasheet for GWB-A43096 | ||||||||||||||||
| COA Datasheet | Printable COA datasheet for GWB-A43096 | ||||||||||||||||
| Storage | Refer to kit instructions for component storage | ||||||||||||||||
| Species Reactivity | Cow | ||||||||||||||||
| Lead Time | Domestic: within 1 week delivery | International: 1 week | ||||||||||||||||
| Intended Use | Research Use Only | ||||||||||||||||
| Additional Information | Intended Use: The Haptoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological samples of cattle. |