Bovine TNF alpha ELISA Reagent Set (GWB-F0F1X9)

GWB-F0F1X9

2 plates
$439.00
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Description

Intended Use The Bovine Tumor necrosis factor TNF alpha ELISA Development Kit is for the quantitative determination of Bovine TNF alpha in cell culture supernatants.
Quantity 2 Plate Set.
Storage 2-8ºC.
Sample Type Cell Culture Supernatants.
Kit Contents
  • Bovine TNF alpha Coated Plate (2 plates)
  • Bovine TNF alpha Standard
  • Bovine TNF alpha Detection Antibody
  • Streptavidin-HRP
ELISA Procedure
  1. Prepare Standard and cell culture supernatant sample dilutions in Standard and Sample Diluent.
  2. Add 100 uL of Standard or sample to appropriate wells.
  3. Note: Run each Standard or sample in duplicate.
  4. Cover plate with Plate Sealer and incubate at room temperature (20-25C) for 1 hour.
  5. Wash plate FOUR times with Wash Buffer.
  6. Note: Gently squeeze the long sides of plate frame before washing to ensure all strips remain securely in the frame. Empty plate contents. Use a squirt wash bottle to vigorously fill each well completely with 1X Wash Buffer, then empty plate contents. Repeat procedure three additional times for a total of FOUR washes. Blot plate onto paper towels or other absorbent material.
  7. Add 100 uL of Detection Antibody Working Solution to each well.
  8. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
  9. Wash plate FOUR times with Wash Buffer as described in step 4.
  10. Add 100 uL of Streptavidin-HPR Working Solution to each well.
  11. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
  12. Wash plate FOUR times with Wash Buffer as described in step 4.
  13. Add 100 uL of TMB Substrate Solution to each well.
  14. Develop the plate in the dark at room temperature for 30 minutes.
  15. Note: Do NOT cover plate with Plate Sealer.
  16. Stop reaction by adding 100 uL of Stop Solution to each well.
  17. Measure absorbance on a plate reader at 450 nm

Additional Information

Name Bovine TNF alpha ELISA Reagent Set (GWB-F0F1X9)
Related Product Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2
Swissprot ID Q06599
Application Quantitative determination of Bovine TNF alpha in cell culture supernatants.
Reactivity Bovine, Cow
Sequence Length 234 AA
Datasheets/Manuals Printable datasheet for GWB-F0F1X9
Intended Use Research Use Only