BPIFA1 ELISA Kit (Human) (GWB-KBBIE3) (Aviva Cat. No. OKCD00535)

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GWB-KBBIE3

Aviva Cat. No.: OKCD00535

Additional Information

Name BPIFA1 ELISA Kit (Human) (GWB-KBBIE3)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BPIFA1
Gene id 51297
Alias Symbols bA49G10.5, LPLUNC3, Lung-specific protein X, LUNX, NASG, Nasopharyngeal carcinoma-related protein, Palate lung and nasal epithelium clone protein, Protein Plunc, Secretory protein in upper respiratory tracts, SPLUNC1, SPURT, Tracheal epithelium-enriched p
Swissprot ID Q9NP55
Protein Name BPI fold-containing family A member 1
Description of Target Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bact
Kit Principle Genway Biotech BPIFA1 ELISA Kit (Human) (GWB-KBBIE3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BPIFA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BPIFA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BPIFA1 captured in the well.
Kit Component
ComponentAmount
Anti-PAK4 Microplate96 Wells (12 x 8 Well Strips)
PAK4 Lyophilized Standard2 x 10 ng
100X Biotinylated PAK4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001230122
Nucleotide Accession Num NM_001243193
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-102%79-101%88-99%78-99%
EDTA Plasma (n=5)80-95%89-102%91-98%91-98%
Heparin Plasma (n=5)91-99%94-103%81-90%89-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105101
EDTA Plasma (n=5)94-10198
Heparin Plasma (n=5)94-10197
Datasheets/Manuals Printable datasheet for GWB-KBBIE3
Species Reactivity Human