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96 Wells
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Additional Information

Name BRAF ELISA Kit (Human) (GWB-KBBBM4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.118 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BRAF
Gene id 673
Alias Symbols BRAF1, B-RAF1, FLJ95109, MGC126806, MGC138284, NS7, p94, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1
Swissprot ID P15056
Protein Name Serine/threonine-protein kinase B-raf
Description of Target Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transducti
Kit Principle Genway Biotech BRAF ELISA Kit (Human) (GWB-KBBBM4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRAF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRAF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BRAF captured in the well.
Kit Component
Anti-BRAF Microplate96 Wells (12 x 8 Well Strips)
BRAF Lyophilized Standard2 x 20 ng
100X Biotinylated BRAF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004324
Nucleotide Accession Num NM_004333
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBM4
Species Reactivity Human