BRINP3 ELISA Kit (Human) (GWB-KBBQT3) (Aviva Cat. No. OKCD01097)

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GWB-KBBQT3

Aviva Cat. No.: OKCD01097

Additional Information

Name BRINP3 ELISA Kit (Human) (GWB-KBBQT3)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.116 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol BRINP3
Gene id 339479
Alias Symbols BRINP3, DBCCR1L, DBCCR1L1, DBCCR1-like protein 1, Protein FAM5C, RP11-445K1.1
Swissprot ID Q76B58
Protein Name BMP/retinoic acid-inducible neural-specific protein 3
Description of Target Inhibits neuronal cell proliferation by negative regulation of the cell cycle transition. Promotes pituitary gonadotrope cell proliferation, migration and invasion, when overexpressed. May play a role in cell pituitary tumor development.
Kit Principle Genway Biotech BRINP3 ELISA Kit (Human) (GWB-KBBQT3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRINP3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRINP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BRINP3 captured in the well.
Kit Component
ComponentAmount
Anti-Msto1 Microplate96 Wells (12 x 8 Well Strips)
Msto1 Lyophilized Standard2 x 20 ng
100X Biotinylated Msto1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001304117
Nucleotide Accession Num NM_001317188
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-104%95-102%82-93%95-102%
EDTA Plasma (n=5)83-101%80-91%85-99%84-96%
Heparin Plasma (n=5)95-104%80-92%90-104%78-91%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9589
EDTA Plasma (n=5)98-105102
Heparin Plasma (n=5)84-10192
Datasheets/Manuals Printable datasheet for GWB-KBBQT3
Species Reactivity Human