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C1qb ELISA Kit (Rat) (GWB-KBBWN7)

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96 Wells
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Additional Information

Name C1qb ELISA Kit (Rat) (GWB-KBBWN7)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.64 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol C1QB
Gene id 29687
Alias Symbols Complement C1q subcomponent subunit B
Swissprot ID P31721
Protein Name Complement C1q subcomponent subunit B
Description of Target C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on int
Kit Principle Genway Biotech C1qb ELISA Kit (Rat) (GWB-KBBWN7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1qb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1qb captured in the well.
Kit Component
Anti-C1qb Microplate96 Wells (12 x 8 Well Strips)
C1qb Lyophilized Standard2 x 100 ng
100X Biotinylated C1qb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_062135
Nucleotide Accession Num NM_019262
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-103%81-102%96-104%95-103%
EDTA Plasma (n=5)85-103%91-102%82-97%84-92%
Heparin Plasma (n=5)98-105%95-105%86-101%96-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-10492
EDTA Plasma (n=5)96-105102
Heparin Plasma (n=5)79-9484
Datasheets/Manuals Printable datasheet for GWB-KBBWN7
Species Reactivity Rat