C1qL1 ELISA Kit (Human) (GWB-KBBZF6) (Aviva Cat. No. OKDD00157)

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GWB-KBBZF6

Aviva Cat. No.: OKDD00157

Additional Information

Name C1qL1 ELISA Kit (Human) (GWB-KBBZF6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.053 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol C1qL1
Gene id 10882
Alias Symbols C1q-related factor, C1QRF, C1QTNF14, Complement component 1 Q subcomponent-like 1, CRF
Swissprot ID O75973
Protein Name C1q-related factor
Description of Target May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses (
Kit Principle Genway Biotech C1qL1 ELISA Kit (Human) (GWB-KBBZF6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1qL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1qL1 captured in the well.
Kit Component
ComponentAmount
Anti-C1qL1 Microplate96 Wells (12 x 8 Well Strips)
C1qL1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X C1qL1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_006679.1
Nucleotide Accession Num NM_006688.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBZF6
Species Reactivity Human