C4A ELISA Kit (Human) (GWB-KBBYJ4)

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96 Wells
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Additional Information

Name C4A ELISA Kit (Human) (GWB-KBBYJ4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.112 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol C4A
Gene id 720
Alias Symbols Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4, C4A2, C4A3, C4A4, C4A6, C4B, C4S, CO4, Complement C4-A, CPAMD2, MGC164979, RG
Swissprot ID P0C0L4
Protein Name Complement C4-A
Description of Target Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of
Kit Principle Genway Biotech C4A ELISA Kit (Human) (GWB-KBBYJ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C4A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C4A captured in the well.
Kit Component
Anti-Esr2 Microplate96 Wells (12 x 8 Well Strips)
Esr2 Lyophilized Standard2 x 20 ng
100X Biotinylated Esr2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001002029
Nucleotide Accession Num NM_001002029
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-105%96-104%79-89%78-88%
EDTA Plasma (n=5)94-101%99-105%97-105%95-102%
Heparin Plasma (n=5)83-90%88-102%84-102%80-91%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-105101
EDTA Plasma (n=5)84-9991
Heparin Plasma (n=5)79-9887
Datasheets/Manuals Printable datasheet for GWB-KBBYJ4
Species Reactivity Human