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C4b ELISA Kit (Mouse) (GWB-KBBFX2)

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96 Wells
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Additional Information

Name C4b ELISA Kit (Mouse) (GWB-KBBFX2)
Kit Range 15.6 - 1,000 ng/mL
Sensitivity < 6.7 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol C4B
Gene id 12268
Alias Symbols C4, Complement C4-B, Ss
Swissprot ID P01029
Protein Name Complement C4-B
Description of Target Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of
Kit Principle Genway Biotech C4b ELISA Kit (Mouse) (GWB-KBBFX2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C4b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C4b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C4b captured in the well.
Kit Component
Anti-C4b Microplate96 Wells (12 x 8 Well Strips)
C4b Lyophilized Standard2 x 1,000 ng
100X Biotinylated C4b Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_033910
Nucleotide Accession Num NM_009780
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-89%92-99%97-105%97-105%
EDTA Plasma (n=5)81-105%96-104%81-103%84-99%
Heparin Plasma (n=5)94-103%82-93%83-92%89-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)85-10496
EDTA Plasma (n=5)95-10298
Heparin Plasma (n=5)99-105102
Datasheets/Manuals Printable datasheet for GWB-KBBFX2
Species Reactivity Mouse