| Name |
C4b ELISA Kit (Mouse) (GWB-KBBFX2) |
| Kit Range |
15.6 - 1,000 ng/mL |
| Sensitivity |
< 6.7 ng/mL |
| Sample Type |
serum, plasma and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
C4B |
| Gene id |
12268 |
| Alias Symbols |
C4, Complement C4-B, Ss |
| Swissprot ID |
P01029 |
| Protein Name |
Complement C4-B |
| Description of Target |
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of |
| Kit Principle |
Genway Biotech C4b ELISA Kit (Mouse) (GWB-KBBFX2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C4b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C4b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C4b captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-C4b Microplate | 96 Wells (12 x 8 Well Strips) |
| C4b Lyophilized Standard | 2 x 1,000 ng |
| 100X Biotinylated C4b Detector Antibody | 120 uL |
| Avidin/HRP Conjugate | 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_033910 |
| Nucleotide Accession Num |
NM_009780 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 78-89% | 92-99% | 97-105% | 97-105% |
| EDTA Plasma (n=5) | 81-105% | 96-104% | 81-103% | 84-99% |
| Heparin Plasma (n=5) | 94-103% | 82-93% | 83-92% | 89-102% |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 85-104 | 96 |
| EDTA Plasma (n=5) | 95-102 | 98 |
| Heparin Plasma (n=5) | 99-105 | 102 |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBFX2 |
| Species Reactivity |
Mouse |
