C6 ELISA Kit (Human) (GWB-KBBIW6) (Aviva Cat. No. OKCD01158)

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Please see item number OKCD01158 to view product details and for ordering information.


GWB-KBBIW6

Aviva Cat. No.: OKCD01158

Additional Information

Name C6 ELISA Kit (Human) (GWB-KBBIW6)
Kit Range 0.625 - 40 ng/mL
Sensitivity < 0.294 ng/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol C6
Gene id 729
Alias Symbols Complement component C6
Swissprot ID P13671
Protein Name complement component C6
Description of Target Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Kit Principle Genway Biotech C6 ELISA Kit (Human) (GWB-KBBIW6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C6 captured in the well.
Kit Component
ComponentAmount
Anti-ALDH1A2 Microplate96 Wells (12 x 8 Well Strips)
ALDH1A2 Lyophilized Standard2 x 40 ng
100X Biotinylated ALDH1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000056
Nucleotide Accession Num NM_000065
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-101%80-90%80-89%83-91%
EDTA Plasma (n=5)78-96%89-104%80-91%95-102%
Heparin Plasma (n=5)84-101%97-104%95-105%98-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-10291
EDTA Plasma (n=5)98-105102
Heparin Plasma (n=5)80-9184
Datasheets/Manuals Printable datasheet for GWB-KBBIW6
Species Reactivity Human