C8B ELISA Kit (Human) (GWB-KBBJM5) (Aviva Cat. No. OKCD00828)

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Please see item number OKCD00828 to view product details and for ordering information.


GWB-KBBJM5

Aviva Cat. No.: OKCD00828

Additional Information

Name C8B ELISA Kit (Human) (GWB-KBBJM5)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.128 ng/mL
Sample Type Plasma
Kit Detection Colorimetric, OD 450 nm
Gene Symbol C8B
Gene id 732
Alias Symbols Complement component 8 subunit beta, Complement component C8 beta chain
Swissprot ID P07358
Protein Name Complement component C8 beta chain
Description of Target Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Kit Principle Genway Biotech C8B ELISA Kit (Human) (GWB-KBBJM5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C8B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C8B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C8B captured in the well.
Kit Component
ComponentAmount
Anti-Ptk2b Microplate96 Wells (12 x 8 Well Strips)
Ptk2b Lyophilized Standard2 x 20 ng
100X Biotinylated Ptk2b Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000057
Nucleotide Accession Num NM_000066
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Sodium Citrate Plasma (n=5)85-96%80-92%93-102%84-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Sodium Citrate Plasma (n=5)90-9995
Datasheets/Manuals Printable datasheet for GWB-KBBJM5
Species Reactivity Human