| Name |
CA5B ELISA Kit (Human) (GWB-KBBXJ5) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.058 ng/mL |
| Sample Type |
Serum, plasma |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
CA5B |
| Gene id |
11238 |
| Alias Symbols |
Carbonate dehydratase VB, Carbonic anhydrase 5B, mitochondrial, Carbonic anhydrase VB, CA-VB, MGC39962 |
| Swissprot ID |
Q9Y2D0 |
| Protein Name |
Carbonic anhydrase 5B, mitochondrial |
| Description of Target |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, |
| Kit Principle |
Genway Biotech CA5B ELISA Kit (Human) (GWB-KBBXJ5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CA5B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CA5B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CA5B captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-CA5B Microplate | 96 Wells (12 x 8 Well Strips) |
| CA5B Lyophilized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X CA5B Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Protein Accession Num |
NP_009151.1 |
| Nucleotide Accession Num |
NM_007220.3 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBXJ5 |
| Species Reactivity |
Human |
