CA5B ELISA Kit (Human) (GWB-KBBXJ5)

GWB-KBBXJ5

96 Wells
$605.00
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Additional Information

Name CA5B ELISA Kit (Human) (GWB-KBBXJ5)
Availability In Stock
Kit Range 0.156-10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type Serum, plasma
Kit Detection Absorbance, OD 450 nm
Gene Symbol CA5B
Gene id 11238
Alias Symbols Carbonate dehydratase VB, Carbonic anhydrase 5B, mitochondrial, Carbonic anhydrase VB, CA-VB, MGC39962
Swissprot ID Q9Y2D0
Protein Name Carbonic anhydrase 5B, mitochondrial
Description of Target Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption,
Kit Principle Genway Biotech CA5B ELISA Kit (Human) (GWB-KBBXJ5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CA5B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CA5B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CA5B captured in the well.
Kit Component
ComponentAmount
Anti-CA5B Microplate96 Wells (12 x 8 Well Strips)
CA5B Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CA5B Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_009151.1
Nucleotide Accession Num NM_007220.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXJ5
Species Reactivity Human