CACNA1D ELISA Kit (Human) (GWB-KBBRQ2)

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GWB-KBBRQ2

96 Wells
$659.00
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Additional Information

Name CACNA1D ELISA Kit (Human) (GWB-KBBRQ2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.121 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CACNA1D
Gene id 776
Alias Symbols CACH3, CACN4, CACNL1A2, Cav1.3, CCHL1A2, Voltage-dependent L-type calcium channel subunit alpha-1D, Voltage-gated calcium channel subunit alpha Cav1.3
Swissprot ID Q01668
Protein Name Voltage-dependent L-type calcium channel subunit alpha-1D
Description of Target Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell mo
Kit Principle Genway Biotech CACNA1D ELISA Kit (Human) (GWB-KBBRQ2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CACNA1D has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CACNA1D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CACNA1D captured in the well.
Kit Component
ComponentAmount
Anti-CACNA1D Microplate96 Wells (12 x 8 Well Strips)
CACNA1D Lyophilized Standard2 x 20 ng
100X Biotinylated CACNA1D Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000711
Nucleotide Accession Num NM_000720
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRQ2
Species Reactivity Human