Cacna1e ELISA Kit (Mouse) (GWB-KBBAK4)

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96 Wells
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Additional Information

Name Cacna1e ELISA Kit (Mouse) (GWB-KBBAK4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CACNA1E
Gene id 12290
Alias Symbols A430040I15, alpha1E, BII, Brain calcium channel II, Cach6, Cacnl1a6, Cav2.3, Cchra1, Voltage-dependent R-type calcium channel subunit alpha-1E, Voltage-gated calcium channel subunit alpha Cav2.3
Swissprot ID Q61290
Protein Name Voltage-dependent R-type calcium channel subunit alpha-1E
Description of Target Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell mo
Kit Principle Genway Biotech Cacna1e ELISA Kit (Mouse) (GWB-KBBAK4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cacna1e has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cacna1e is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cacna1e captured in the well.
Kit Component
Anti-Cacna1e Microplate96 Wells (12 x 8 Well Strips)
Cacna1e Lyophilized Standard2 x 20 ng
100X Biotinylated Cacna1e Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_033912
Nucleotide Accession Num NM_009782
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAK4
Species Reactivity Mouse