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96 Wells
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Additional Information

Name CALML3 ELISA Kit (Human) (GWB-KBBON6)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CALML3
Gene id 810
Alias Symbols Calmodulin-like protein 3, Calmodulin-related protein NB-1, CaM-like protein, CLP
Swissprot ID P27482
Protein Name calmodulin-like protein 3
Description of Target May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, t
Kit Principle Genway Biotech CALML3 ELISA Kit (Human) (GWB-KBBON6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALML3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CALML3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALML3 captured in the well.
Kit Component
Anti-SYNE2 Microplate96 Wells (12 x 8 Well Strips)
SYNE2 Lyophilized Standard2 x 10 ng
100X Biotinylated SYNE2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_005176
Nucleotide Accession Num NM_005185
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBON6
Species Reactivity Human